EVALUATION OF THE PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF THE FRACTIONATED METHANOLIC LEAF EXTRACT OF DIOSPYROS PRUESSII GURKE.

The methanolic leaf extract of D. pruessii with documented ethnomedicinal applications was fractionated in different solvents (n-hexane, chloroform, ethyl acetate, n-butanol, and water) subjected to phytochemical screening antimicrobial activity against some disease-causing microorganisms including gram-positive gram-negative (Staphylococcus aureus, salmonella spp, Escherichia coli). Phytochemical revealed the presence Alkaloids, saponins, saponin glycosides, Tannins, hydrolyzable steroids triterpenoids, flavonoids, phenols, volatile oils. Each fraction demonstrated antibacterial all organisms tested. MIC values each strong inhibition Pruessii water, n-hexane, n-butanol showed following values: Staphylococcus A. 12.5, 25, 50, 12.5. E.coli 100, 50 100. Salmonella spp 3.125, respectively. tests were carried out triplicates, data obtained one-way ANOVA using a statistical package for social science (SPSS). This study shows that these plants' possesses properties can be used as alternatives conventional antibiotics.